An optimized one-tube, semi-nested PCR assay for Paracoccidioides brasiliensis detection

An optimized one-tube, semi-nested PCR assay for Paracoccidioides brasiliensis detection

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Introduction Herein, we report a one-tube, semi-nested-polymerase chain reaction (OTsn-PCR) assay for the detection of Paracoccidioides brasiliensis.Methods We developed the OTsn-PCR KIDWEAR assay for the detection of P.brasiliensis in clinical specimens and compared it with other PCR methods.Results The OTsn-PCR assay was positive for all clinical samples, and the detection limit was better or equivalent to the other nested or semi-nested PCR methods Lamps for P.

brasiliensis detection.Conclusions The OTsn-PCR assay described in this paper has a detection limit similar to other reactions for the molecular detection of P.brasiliensis, but this approach is faster and less prone to contamination than other conventional nested or semi-nested PCR assays.